Date of publication: 2017-07-08 16:26
When the enzyme and substrate form a complex, structural changes occur so that the active site fits precisely around the substrate (the substrate induces the active site to change shape).
The pH must be correct for each enzyme. If the conditions are too alkaline or acidic then the activity of the enzyme is affected. This happens because the enzyme s shape, especially the active site, is changed. It is denatured , and cannot hold the substrate molecule.
If collecting the gas over water is complicated, and you have access to a 655 cm 8 gas syringe, you could collect the gas in that instead. Be sure to clamp the gas syringe securely but carefully.
The site of the reaction occurs in an area on the surface of the protein called the active site. Since the active site for all molecules of one enzyme will be made up of the same arrangement of amino acids, it has a highly specific shape.
When more substrate molecules are added, more enzyme-substrate complexes can be formed. As there are more active sites, and the rate of reaction increases.
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